![]() ![]() ![]() Using an automated cryocooling instrument developed for cryocrystallography that combines high plunge speeds with efficient removal of cold gas, we show that single-particle cryo-EM samples on commercial grids can be routinely vitrified using only boiling nitrogen and obtain apoferritin datasets and refined structures with 2.65 Å resolution. The use of liquid nitrogen as the primary coolant may allow manual and automated workflows to be simplified and may reduce sample stresses that contribute to beam-induced motion. ![]() Single-particle cryo-electron microscopy (cryo-EM) (Frank, 2002 ) has emerged as a powerful approach to obtaining near atomic resolution structures of large biomolecular complexes, membrane proteins, and other targets of major scientific, pharmaceutical and biotechnological interest (Cheng, 2015, 2018 Glaeser, 2016 b, 2019 Vinothkumar & Henderson, 2016 Lyumkis, 2019 ). #Make visuals great again v 2.65 manual#. ![]()
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |